In normal operation, there should be +300 VDC or more measured from either 80 tube filament pin to chassis ground. However...
A negative DC voltage will be observed if you measure from either plate to ground. The center tap of the B+ winding is referenced to chassis ground through the 325 ohm power resistor. With the diode action this creates a negative DC voltage on the center tap node (and both plates, as well). This negative voltage is used to create tube grid bias voltages; -65 volts seems a bit excessive- I'd expect only a dozen volts or so. You might check that the 325 ohm wirewound resistor isn't open. If it is, it would knock out normal B+ and may account for the "high" negative voltage (since your voltmeter impedance is serving as a high-resistance return path).
So... my next guess is that the HT winding center tap wire from the transformer to this resistor has opened up. What is the DC voltage on the CT end of this resistor?